**Pepetools** is a bioinformatics tool used for the analysis of protein and peptide sequences. It assists researchers in examining various properties of peptides, such as molecular weight, amino acid composition, hydrophobicity, and isoelectric point. By inputting a peptide or protein sequence, users can gain quick insights into the physicochemical characteristics that influence structure and function. Peptools is especially valuable in protein engineering, drug design, and peptide synthesis, where understanding sequence behavior is critical. It also helps in identifying motifs or conserved regions within proteins, aiding in functional annotation and evolutionary studies. The tool is often used by molecular biologists, biochemists, and bioinformaticians for both academic and industrial research. With a user-friendly interface and fast processing capabilities, Peptools simplifies complex calculations, saving time and enhancing productivity. Overall, it serves as a reliable companion in peptide research and design, bridging the gap between theoretical data and practical laboratory applications.